EMD-2511

Single-particle
7.4 Å
EMD-2511
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Deposition: 19/11/2013
Map released: 05/02/2014
Last modified: 10/09/2014
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2511
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4cg5 summary report (pdf.gz) Map and 4cg5 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM of the Sec61-complex bound to an 80S-RNC engaged with a translocating LepT chain

EMD-2511

Single-particle
7.4 Å
EMD-2511
3D View Gallery
Deposition: 19/11/2013
Map released: 05/02/2014
Last modified: 10/09/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.6
Details: 30 mM Hepes/KOH 7.6, 10 mM Mg(OAc)2, 180 mM KOAC/HAc pH 7.6, 0.3 % Digitonin, 1 mM DTT
Grid
Details: Quantifoil grids pre-coated with 2 nm carbon on top
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Instrument: FEI VITROBOT MARK IV
Method: Blot for 3 seconds before plunging
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.3 µm - 4.0 µm
Calibrated magnification: 148721.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Number of real images: 9805
Average electron dose per image: 25 e/Å2
Bits per pixel: 16.0
Image processing [1]
Details: The particles were selected using SIGNATURE, classified using MAPPOS and processed with SPIDER
Final reconstruction
Resolution: 7.4 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 53248
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER, Signature, MAPPOS - -
CTF correction
Details:On 3D-volume (SPIDER)
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of 80S-RNC-Sec61 complex engaged with translocating nascent chain (LepT)
Details: ::::EMDATABANK.org::::EMD-2511::::
⬡ Geometry
X Y Z
Dimensions 368 368 368
Origin -184 -184 -183
Spacing 368 368 368
Voxel size 1.2374 Å 1.2374 Å 1.2374 Å
Contour list
Primary Level Source
True 0.1 AUTHOR