EMD-2521

Subtomogram averaging
45.0 Å
EMD-2521 Deposition: 26/11/2013
Map released: 25/12/2013
Last modified: 25/12/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2521

Flagellar hook basal body in the tomogram of Salmonella mini-cell

EMD-2521

Subtomogram averaging
45.0 Å
EMD-2521 Deposition: 26/11/2013
Map released: 25/12/2013
Last modified: 25/12/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Subtomogram averaging
Aggregation State: Cell
Specimen preparation [1]
Buffer
Details: M9 medium (17.1g Na2HPO4-12H2O, 3g KH2PO4, 0.5g NaCl, 1g NH4Cl, 0.2%glycerol, 1% tryptone, 1mM MgSO4 per litre)
Grid
Details: Quantifoil molybdenum 200 mesh R0.6/1.0 grid with thin carbon support
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK II
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 4.0 µm - 7.0 µm
Nominal magnification: 29000.0
Calibrated magnification: 49030.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Temperature
Minimum: 80 K
Image Recording [1]
Detector category: CCD
Detector model: FEI FALCON I (4k x 4k)
Average electron dose per image: 200 e/Å2
Tilt Series [1]
Axis 1 Axis 2
Min. Max. Inc. Min. Max. Inc. Rotation
-70 ° 70 ° - - - - -
Image processing [1]
Final reconstruction
Resolution: 45.0 Å ( BY AUTHOR)
Resolution method: OTHER
Algorithm: OTHER
Applied Symmetry
Point group: C26
Software [1]
Name Version Details
EMAN - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Flagellar hook basal body from Salmonella mini-cell
Details: ::::EMDATABANK.org::::EMD-2521::::
Geometry
X Y Z
Dimensions 82 82 82
Origin -234 677 -49
Spacing 82 82 82
Voxel size 11.4 Å 11.4 Å 11.4 Å
Contour list
Primary Level Source
True 0.0952 AUTHOR