EMD-2521
Flagellar hook basal body in the tomogram of Salmonella mini-cell
EMD-2521
Subtomogram averaging45.0 Å
Deposition: 26/11/2013
Map released: 25/12/2013
Last modified: 25/12/2013
Buffer
Details: M9 medium (17.1g Na2HPO4-12H2O, 3g KH2PO4, 0.5g NaCl, 1g NH4Cl, 0.2%glycerol, 1% tryptone, 1mM MgSO4 per litre)
Grid
Details: Quantifoil molybdenum 200 mesh R0.6/1.0 grid with thin carbon support
Vitrification
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 4.0 µm - 7.0 µm
Nominal magnification: 29000.0
Calibrated magnification: 49030.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 4.0 µm - 7.0 µm
Nominal magnification: 29000.0
Calibrated magnification: 49030.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Temperature
Minimum: 80
K
Image Recording
[1]
Detector category:
CCD
Detector model: FEI FALCON I (4k x 4k)
Average electron dose per image: 200 e/Å2
Detector model: FEI FALCON I (4k x 4k)
Average electron dose per image: 200 e/Å2
Tilt Series
[1]
Axis 1 | Axis 2 | |||||
---|---|---|---|---|---|---|
Min. | Max. | Inc. | Min. | Max. | Inc. | Rotation |
-70 ° | 70 ° | - | - | - | - | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Flagellar hook basal body from Salmonella mini-cell
Details: ::::EMDATABANK.org::::EMD-2521::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Flagellar hook basal body from Salmonella mini-cell
Details: ::::EMDATABANK.org::::EMD-2521::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 82 | 82 | 82 |
Origin | -234 | 677 | -49 |
Spacing | 82 | 82 | 82 |
Voxel size | 11.4 Å | 11.4 Å | 11.4 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.0952 | AUTHOR |