EMD-2555

Single-particle
18.0 Å
EMD-2555 Deposition: 16/01/2014
Map released: 14/05/2014
Last modified: 25/06/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2555

Negative-stain electron microscopy of E. coli ClpB mutant E432A (BAP form bound to ClpP)

EMD-2555

Single-particle
18.0 Å
EMD-2555 Deposition: 16/01/2014
Map released: 14/05/2014
Last modified: 25/06/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.03 mg/mL
Buffer
pH: 7.5
Details: 20 mM Tris-HCl, pH 7.5, 20 mM KCl, 15 mM MgCl2, 1 mM DTT, 2 mM ATPgammaS
Staining
Type: NEGATIVE
Details: Protein adsorbed on carbon coated grids pretreated with 0.01% poly lysine chains. Stained with 2% uranyl acetate for 1 minute.
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 0.5 µm - 1.2 µm
Nominal magnification: 50000.0
Calibrated magnification: 68000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 150,000 x magnification, Electron beam tilt params: )
Image Recording [1]
Detector category: CCD
Detector model: GATAN K2 (4k x 4k)
Number of real images: 110
Average electron dose per image: 20 e/Å2
Image processing [1]
Final reconstruction
Resolution: 18.0 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 11570
Algorithm: OTHER
Applied Symmetry
Point group: C6
Software [1]
Name Version Details
IMAGIC, Spider - -
Final angle assignment
Details: Only side views used. Beta angles between 80 and 100 degrees (IMAGIC convention).
CTF correction
Details:Phase flipping entire frame
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of ClpB E432A mutant (BAP form bound to ClpP). Six-fold symmetry applied.
Details: ::::EMDATABANK.org::::EMD-2555::::
Geometry
X Y Z
Dimensions 256 256 256
Origin -127 -127 -127
Spacing 256 256 256
Voxel size 2.0 Å 2.0 Å 2.0 Å
Contour list
Primary Level Source
True 0.168 AUTHOR