EMD-2555
Negative-stain electron microscopy of E. coli ClpB mutant E432A (BAP form bound to ClpP)
EMD-2555
Single-particle18.0 Å
Deposition: 16/01/2014
Map released: 14/05/2014
Last modified: 25/06/2014
Concentration: 0.03
mg/mL
Buffer
pH: 7.5
Details: 20 mM Tris-HCl, pH 7.5, 20 mM KCl, 15 mM MgCl2, 1 mM DTT, 2 mM ATPgammaS
Details: 20 mM Tris-HCl, pH 7.5, 20 mM KCl, 15 mM MgCl2, 1 mM DTT, 2 mM ATPgammaS
Staining
Type:
NEGATIVE
Details: Protein adsorbed on carbon coated grids pretreated with 0.01% poly lysine chains. Stained with 2% uranyl acetate for 1 minute.
Details: Protein adsorbed on carbon coated grids pretreated with 0.01% poly lysine chains. Stained with 2% uranyl acetate for 1 minute.
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 0.5 µm - 1.2 µm
Nominal magnification: 50000.0
Calibrated magnification: 68000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 150,000 x magnification, Electron beam tilt params: )
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 0.5 µm - 1.2 µm
Nominal magnification: 50000.0
Calibrated magnification: 68000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 150,000 x magnification, Electron beam tilt params: )
Image Recording
[1]
Detector category:
CCD
Detector model: GATAN K2 (4k x 4k)
Number of real images: 110
Average electron dose per image: 20 e/Å2
Detector model: GATAN K2 (4k x 4k)
Number of real images: 110
Average electron dose per image: 20 e/Å2
Final
reconstruction
Resolution: 18.0
Å
(
BY AUTHOR)
Resolution method: OTHER
Number of images used: 11570
Algorithm: OTHER
Resolution method: OTHER
Number of images used: 11570
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
C6
Software
[1]
Name | Version | Details |
---|---|---|
IMAGIC, Spider | - | - |
⦩ Final angle assignment
Details: Only side views used. Beta angles between 80 and 100 degrees (IMAGIC convention).
CTF correction
Details:Phase flipping entire frame
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of ClpB E432A mutant (BAP form bound to ClpP). Six-fold symmetry applied.
Details: ::::EMDATABANK.org::::EMD-2555::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of ClpB E432A mutant (BAP form bound to ClpP). Six-fold symmetry applied.
Details: ::::EMDATABANK.org::::EMD-2555::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 256 | 256 | 256 |
Origin | -127 | -127 | -127 |
Spacing | 256 | 256 | 256 |
Voxel size | 2.0 Å | 2.0 Å | 2.0 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.168 | AUTHOR |