EMD-2984

Single-particle
2.2 Å
EMD-2984
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Deposition: 26/04/2015
Map released: 06/05/2015
Last modified: 14/10/2015
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EMD-2984
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5a1a summary report (pdf.gz) Map and 5a1a FULL report (pdf.gz)
EMICSS annotations (xml)

2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor

EMD-2984

Single-particle
2.2 Å
EMD-2984
3D View Gallery
Deposition: 26/04/2015
Map released: 06/05/2015
Last modified: 14/10/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 2.3 mg/mL
Buffer
pH: 8.0
Details: 25 mM Tris, pH 8.0, 50 mM NaCl, 2 mM MgCl2, 1.0 mM TCEP
Grid
Details: 200 mesh Quantifoil R2/2 grids, plasma cleaned
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 90.15 K
Instrument: LEICA EM GP
Method: Blot for 2 seconds before plunging.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.6 µm - 2.0 µm
Nominal magnification: 215000.0
Calibrated magnification: 215000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Specimen holder details: Liquid nitrogen cooled
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 215,000 times magnification., Electron beam tilt params: )
Details: Parallel beam illumination
Temperature
Minimum: 79.6 K
Average: 79.7 K
Maximum: 79.8 K
Specialist optics
Energy filter
Name: Gatan, Inc.
Lower energy threshold: 0.0 eV
Upper energy threshold: 20.0 eV
Image Recording [1]
Detector category: CCD
Detector model: GATAN K2 (4k x 4k)
Number of real images: 1487
Average electron dose per image: 45 e/Å2
Details: Every image is the average of 38 frames recorded by the direct electron detector.
Image processing [1]
Final reconstruction
Resolution: 2.2 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 41123
Algorithm: OTHER
⌯ Applied Symmetry
Point group: D2
Software [1]
Name Version Details
FREALIGN - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: B-factor corrected reconstruction of PETG-bound beta-galactosidase.
Details: ::::EMDATABANK.org::::EMD-2984::::
⬡ Geometry
X Y Z
Dimensions 292 292 292
Origin 0 0 0
Spacing 292 292 292
Voxel size 0.637 Å 0.637 Å 0.637 Å
Contour list
Primary Level Source
True 0.05 AUTHOR