EMD-5119

Single-particle
7.9 Å
EMD-5119 Deposition: 08/06/2009
Map released: 30/09/2009
Last modified: 05/05/2010
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5119

Clathrin D6 coat

EMD-5119

Single-particle
7.9 Å
EMD-5119 Deposition: 08/06/2009
Map released: 30/09/2009
Last modified: 05/05/2010
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 6.5
Details: 25mM MES pH 6.5, 2mM DTT
Grid
Details: Holey carbon grid
Vitrification
Cryogen name: ETHANE
Chamber temperature: 93 K
Instrument: OTHER
Details: Vitrification instrument: FEI Vitrobot
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 2.0 µm - 5.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 51159.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side entry liquid nitrogen-cooled cryo specimen holder
Temperature
Average: 93 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 225
Old range: 1.4
Bits per pixel: 8.0
Image processing [1]
Final reconstruction
Resolution: 7.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 1450
Algorithm: OTHER
Applied Symmetry
Point group: D6
Software [1]
Name Version Details
Frealign - -
CTF correction
Details:CTF correction of each particle.
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This is an averaged map of clathrin D6 coat
Details: ::::EMDATABANK.org::::EMD-5119::::
Geometry
X Y Z
Dimensions 312 312 312
Origin -156 -156 -156
Spacing 312 312 312
Voxel size 2.8 Å 2.8 Å 2.8 Å
Contour list
Primary Level Source
True 0.25 EMDB