EMD-5129

Helical reconstruction
EMD-5129 Deposition: 06/08/2009
Map released: 17/09/2009
Last modified: 02/04/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5129

Reconstruction of ParM in the open state using cryo-EM

EMD-5129

Helical reconstruction
EMD-5129 Deposition: 06/08/2009
Map released: 17/09/2009
Last modified: 02/04/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Vitrification
Cryogen name: ETHANE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: 626
Image processing [1]
Final reconstruction
Applied Symmetry
ΔΖ: 24.2 Å
ΔΦ: 165°
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of ParM in the open state
Details: ::::EMDATABANK.org::::EMD-5129::::
Geometry
X Y Z
Dimensions 100 100 200
Origin -50 -50 -100
Spacing 100 100 200
Voxel size 2.38 Å 2.38 Å 2.38 Å
Contour list
Primary Level Source
True 1000.0 AUTHOR