EMD-5130

Single-particle
7.5 Å
EMD-5130
3D View Gallery
Deposition: 18/08/2009
Map released: 21/12/2009
Last modified: 23/09/2011
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5130
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

3D cryo-EM Structure of Archaeal 20S Proteasome in Complex with the C-terminus of PAN

EMD-5130

Single-particle
7.5 Å
EMD-5130
3D View Gallery
Deposition: 18/08/2009
Map released: 21/12/2009
Last modified: 23/09/2011
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.3 mg/mL
Buffer
pH: 8.5
Details: 50 mM Tris pH 8.5, 1mM DTT, 10 mM MgCl2, 5% Glycerol
Grid
Details: 400 mesh Cu grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 90 K
Instrument: OTHER
Method: blot for 4 seconds before plunging
Details: Vitrification instrument: Vitrobot
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.0 µm - 5.0 µm
Nominal magnification: 62000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Gatan CT-3500
Minimum tilt angle: 0
Maximum tilt angle: 0
Details: Data was collected using Leginon
Temperature
Minimum: 90 K
Average: 90 K
Maximum: 90 K
Image Recording [1]
Detector category: CCD
Detector model: GENERIC GATAN (4k x 4k)
Average electron dose per image: 20 e/Å2
Details: Images were recorded on CCD camera
Image processing [1]
Details: The particles were manually selected
Final reconstruction
Resolution: 7.5 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 13020
Algorithm: OTHER
Details: D7 symmetry was applied
⌯ Applied Symmetry
Point group: D7
Software [1]
Name Version Details
FREALIGN - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This is the 3D volume of T.acidophilum 20S proteasome in complex with a hybrid activator of PA26 and PAN
Details: ::::EMDATABANK.org::::EMD-5130::::
⬡ Geometry
X Y Z
Dimensions 116 116 196
Origin -58 -58 -98
Spacing 116 116 196
Voxel size 1.79103 Å 1.79103 Å 1.79102 Å
Contour list
Primary Level Source
True 1.3 AUTHOR