EMD-5294

Single-particle
9.6 Å
EMD-5294
3D View Gallery
Deposition: 22/05/2011
Map released: 29/05/2012
Last modified: 29/05/2012
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EMD-5294
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3D reconstruction of frozen hydrated HIV-1 integrase dimer in complex with two Fabs.

EMD-5294

Single-particle
9.6 Å
EMD-5294
3D View Gallery
Deposition: 22/05/2011
Map released: 29/05/2012
Last modified: 29/05/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Grid
Details: 200 mesh Quantifoil
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 100 K
Instrument: OTHER
Details: Vitrification instrument: Vitrobot
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.1 mm
Nominal defocus: 2.0 µm - 5.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: CT3500
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F816 (8k x 8k)
Average electron dose per image: 30 e/Å2
Image processing [1]
Final reconstruction
Resolution: 9.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 14000
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C2
Software [1]
Name Version Details
Frealign - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This is 3D reconstruction of frozen hydrated HIV-1 integrase dimer in complex with 2 Fabs.
Details: ::::EMDATABANK.org::::EMD-5294::::
⬡ Geometry
X Y Z
Dimensions 148 148 148
Origin -54 -74 -74
Spacing 148 148 148
Voxel size 1.8 Å 1.8 Å 1.8 Å
Contour list
Primary Level Source
True 1.0 AUTHOR