EMD-5295

Single-particle
25.0 Å
EMD-5295
3D View Gallery
Deposition: 23/05/2011
Map released: 29/05/2012
Last modified: 29/05/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5295
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3D reconstruction of negatively stained PCSK9 in complex with a Fab

EMD-5295

Single-particle
25.0 Å
EMD-5295
3D View Gallery
Deposition: 23/05/2011
Map released: 29/05/2012
Last modified: 29/05/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Staining
Type: NEGATIVE
Details: Uranyl formate
Grid
Details: 200 mesh copper grid
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 2.0 mm
Nominal defocus: 0.0015 µm - 0.0015 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Specimen holder details: Single tilt
Alignment procedure: LEGACY (Astigmatism: each particle, Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 60
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Average electron dose per image: 30 e/Å2
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 3876
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Frealign - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D reconstruction of negatively stained PCSK9-Fab complex
Details: ::::EMDATABANK.org::::EMD-5295::::
⬡ Geometry
X Y Z
Dimensions 60 60 60
Origin 0 0 0
Spacing 60 60 60
Voxel size 4.26 Å 4.26 Å 4.26 Å
Contour list
Primary Level Source
True 0.5 AUTHOR