EMD-5327
Cryo EM reconstruction of mammalian 80S ribosome in classic PRE state 2
EMD-5327
Single-particle9.0 Å
Deposition: 12/07/2011
Map released: 09/11/2011
Last modified: 06/03/2013
Microscope: FEI POLARA 300
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 2.0 µm - 4.0 µm
Nominal magnification: 39000.0
Calibrated magnification: 65520.0
Specimen holder model: GATAN HELIUM
Specimen holder details: Cartridge
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 200,000 times magnification, Electron beam tilt params: )
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 2.0 µm - 4.0 µm
Nominal magnification: 39000.0
Calibrated magnification: 65520.0
Specimen holder model: GATAN HELIUM
Specimen holder details: Cartridge
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 200,000 times magnification, Electron beam tilt params: )
Temperature
Average: 77
K
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: PRIMESCAN
Sampling interval: 4.7 µm
Number of real images: 87
Average electron dose per image: 20 e/Å2
Detector model: KODAK SO-163 FILM
Scanner: PRIMESCAN
Sampling interval: 4.7 µm
Number of real images: 87
Average electron dose per image: 20 e/Å2
Final
reconstruction
Resolution: 9.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 81946
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 81946
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
SPIDER | - | - |
CTF correction
Details:By defocus groups
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 80S mammalian ribosome
Details: ::::EMDATABANK.org::::EMD-5327::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 80S mammalian ribosome
Details: ::::EMDATABANK.org::::EMD-5327::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 180 | 180 | 180 |
Origin | -90 | -90 | -89 |
Spacing | 180 | 180 | 180 |
Voxel size | 2.52 Å | 2.52 Å | 2.52 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 1000.0 | AUTHOR |