EMD-5327

Single-particle
9.0 Å
EMD-5327 Deposition: 12/07/2011
Map released: 09/11/2011
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5327

Cryo EM reconstruction of mammalian 80S ribosome in classic PRE state 2

EMD-5327

Single-particle
9.0 Å
EMD-5327 Deposition: 12/07/2011
Map released: 09/11/2011
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Grid
Details: Quantifoil grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 77 K
Instrument: OTHER
Method: Flash-frozen in liquid ethane on carbon coated Quantifoil grids
Details: Vitrification instrument: VitroBot (FEI)
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 2.0 µm - 4.0 µm
Nominal magnification: 39000.0
Calibrated magnification: 65520.0
Specimen holder model: GATAN HELIUM
Specimen holder details: Cartridge
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 200,000 times magnification, Electron beam tilt params: )
Temperature
Average: 77 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: PRIMESCAN
Sampling interval: 4.7 µm
Number of real images: 87
Average electron dose per image: 20 e/Å2
Image processing [1]
Final reconstruction
Resolution: 9.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 81946
Algorithm: OTHER
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
CTF correction
Details:By defocus groups
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 80S mammalian ribosome
Details: ::::EMDATABANK.org::::EMD-5327::::
Geometry
X Y Z
Dimensions 180 180 180
Origin -90 -90 -89
Spacing 180 180 180
Voxel size 2.52 Å 2.52 Å 2.52 Å
Contour list
Primary Level Source
True 1000.0 AUTHOR