EMD-5342

Single-particle
EMD-5342 Deposition: 25/09/2011
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5342

DNA-free p53 Tetramer Reconstruction

EMD-5342

Single-particle
EMD-5342 Deposition: 25/09/2011
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 7.0
Details: 150mM NaCl
Staining
Type: NEGATIVE
Details: 1% uranyl formate
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI SPHERA
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 200 kV
Specimen holder model: PHILIPS ROTATION HOLDER
Specimen holder details: Eucentric
Image Recording [1]
Number of real images: 50
Image processing [1]
Final reconstruction
Number of images used: 8550
Applied Symmetry
Point group: C1
Final 2D classification
Number of classes: 50
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: DNA-free p53 Tetramer Reconstruction
Details: ::::EMDATABANK.org::::EMD-5342::::
Geometry
X Y Z
Dimensions 120 120 120
Origin -60 -60 -60
Spacing 120 120 120
Voxel size 2.2 Å 2.2 Å 2.2 Å
Contour list
Primary Level Source
True 0.157 AUTHOR