EMD-5379

Single-particle
19.0 Å
EMD-5379
3D View Gallery
Deposition: 06/01/2012
Map released: 13/02/2013
Last modified: 14/08/2013
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5379
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Cryo-EM 3D reconstruction of naturally empty Triatoma Virus

EMD-5379

Single-particle
19.0 Å
EMD-5379
3D View Gallery
Deposition: 06/01/2012
Map released: 13/02/2013
Last modified: 14/08/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Details: NMT (10 mM NaCl, 1 mM MgCl2, 50 mM Tris-HCl)
Grid
Details: 400 mesh quantifoil grid
Vitrification
Cryogen name: ETHANE
Instrument: OTHER
Details: Vitrification instrument: Vitrobot FEI
Microscopy [1]
Microscope: FEI/PHILIPS CM200T
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.2 µm - 4.0 µm
Nominal magnification: 38000.0
Calibrated magnification: 39500.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side entry liquid nitrogen-cooled cryo specimen holder
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 15
Image processing [1]
Final reconstruction
Resolution: 19.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 695
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EM3DR2 - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D reconstruction of naturally empty TrV particle
Details: ::::EMDATABANK.org::::EMD-5379::::
⬡ Geometry
X Y Z
Dimensions 255 255 255
Origin -107 -127 -127
Spacing 255 255 255
Voxel size 1.77 Å 1.77 Å 1.77 Å
Contour list
Primary Level Source
True 0.001 AUTHOR