EMD-5387

Single-particle
25.0 Å
EMD-5387
3D View Gallery
Deposition: 30/01/2012
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5387
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Structure of the Fab-Labeled 'Breathing' State of Native Poliovirus

EMD-5387

Single-particle
25.0 Å
EMD-5387
3D View Gallery
Deposition: 30/01/2012
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 20 mM Tris, 2 mM CaCl2
Vitrification
Cryogen name: ETHANE
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TECNAI F30
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 2.395 µm - 3.037 µm
Nominal magnification: 39000.0
Calibrated magnification: 39000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: Bsoft, Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 7 µm
Number of real images: 7
Average electron dose per image: 10 e/Å2
Bits per pixel: 8.0
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 137
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EM3DR2 - -
CTF correction
Details:CTF and decay correct
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of the Fab-Labeled 'Breathing' State of Native Poliovirus
Details: ::::EMDATABANK.org::::EMD-5387::::
⬡ Geometry
X Y Z
Dimensions 267 267 267
Origin -133 -133 -133
Spacing 267 267 267
Voxel size 1.63 Å 1.63 Å 1.63 Å
Contour list
Primary Level Source
True 5.3 AUTHOR