EMD-5412

Single-particle
11.4 Å
EMD-5412
3D View Gallery
Deposition: 18/04/2012
Map released: 13/02/2013
Last modified: 20/02/2013
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5412
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

CryoEM icosahedral reconstruction of AHSV7

EMD-5412

Single-particle
11.4 Å
EMD-5412
3D View Gallery
Deposition: 18/04/2012
Map released: 13/02/2013
Last modified: 20/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.5
Details: 2mM Tris-HCl
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 0.8 µm - 5.0 µm
Nominal magnification: 83000.0
Calibrated magnification: 83000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Detector category: CCD
Detector model: GENERIC CCD
Number of real images: 507
Average electron dose per image: 18 e/Å2
Image processing [1]
Final reconstruction
Resolution: 11.4 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 2798
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
AUTO3DEM - -
CTF correction
Details:Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: AHSV7 truncated VP2 filled
Details: ::::EMDATABANK.org::::EMD-5412::::
⬡ Geometry
X Y Z
Dimensions 513 513 513
Origin -256 -256 -256
Spacing 513 513 513
Voxel size 1.8 Å 1.8 Å 1.8 Å
Contour list
Primary Level Source
True 1.0 AUTHOR