EMD-5444

Helical reconstruction
20.0 Å
EMD-5444
3D View Gallery
Deposition: 04/07/2012
Map released: 24/10/2012
Last modified: 17/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5444
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

MDA5-dsRNA filament

EMD-5444

Helical reconstruction
20.0 Å
EMD-5444
3D View Gallery
Deposition: 04/07/2012
Map released: 24/10/2012
Last modified: 17/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: TUNGSTEN HAIRPIN
Acceleration voltage: 80 kV
Specimen holder model: OTHER
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON COOLSCAN
Sampling interval: 4.16 µm
Bits per pixel: 14.0
Image processing [1]
Details: The particles were processed using IHRSR
Final reconstruction
Resolution: 20.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
⌯ Applied Symmetry
ΔΖ: 43.6 Å
ΔΦ: 82.7°
Software [1]
Name Version Details
Spider - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of MDA5-dsRNA
Details: ::::EMDATABANK.org::::EMD-5444::::
⬡ Geometry
X Y Z
Dimensions 100 100 100
Origin 0 0 0
Spacing 100 100 100
Voxel size 4.16 Å 4.16 Å 4.16 Å
Contour list
Primary Level Source
True 0.175 EMDB