EMD-5465

Single-particle
6.3 Å
EMD-5465
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Deposition: 09/08/2012
Map released: 03/04/2013
Last modified: 17/04/2013
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EMD-5465
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EMICSS annotations (xml)

Cryo-electron microscopy reconstruction of the Enterovirus 71 A-particle

EMD-5465

Single-particle
6.3 Å
EMD-5465
3D View Gallery
Deposition: 09/08/2012
Map released: 03/04/2013
Last modified: 17/04/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.1 mg/mL
Buffer
pH: 7.5
Details: 10 mM Tris-HCl, 20 mM NaCl, 5 mM MgCl2
Grid
Details: Glow discharged holey carbon Quantifoil electron microscopy grids
Vitrification
Cryogen name: ETHANE-PROPANE MIXTURE
Chamber humidity: 95%
Chamber temperature: 76 K
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.73 µm - 4.28 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: CTFFIND3 (EMAN), Electron beam tilt params: )
Temperature
Average: 95 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 40
Average electron dose per image: 15 e/Å2
Image processing [1]
Details: The particles were selected using an automatic selection program (EMAN).
Final reconstruction
Resolution: 6.3 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 11436
Algorithm: OTHER
Details: A single data set was used for the reconstruction
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EMAN2, auto3DEM - -
CTF correction
Details:Auto3DEM
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the Enterovirus 71 A-particle, produced by heat treating mature capsids in isotonic solution.
Details: ::::EMDATABANK.org::::EMD-5465::::
⬡ Geometry
X Y Z
Dimensions 351 351 351
Origin -175 -175 -175
Spacing 351 351 351
Voxel size 1.27 Å 1.27 Å 1.27 Å
Contour list
Primary Level Source
True 1.0 AUTHOR