EMD-5466
Cryo-electron microscopy reconstruction of the Enterovirus 71 empty capsid
EMD-5466
Single-particle9.2 Å
Deposition: 10/08/2012
Map released: 03/04/2013
Last modified: 17/04/2013
Concentration: 0.1
mg/mL
Buffer
pH: 7.5
Details: 10 mM Tris-HCl, 20 mM NaCl, 5 mM MgCl2
Details: 10 mM Tris-HCl, 20 mM NaCl, 5 mM MgCl2
Grid
Details: Glow discharged holey carbon Quantifoil electron microscopy grids
Vitrification
Cryogen name: ETHANE-PROPANE MIXTURE
Chamber humidity: 95%
Chamber temperature: 77 K
Instrument: FEI VITROBOT MARK III
Chamber humidity: 95%
Chamber temperature: 77 K
Instrument: FEI VITROBOT MARK III
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.73 µm - 4.37 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: CTFFIND3 (EMAN), Electron beam tilt params: )
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.73 µm - 4.37 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: CTFFIND3 (EMAN), Electron beam tilt params: )
Temperature
Average: 95
K
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 40
Average electron dose per image: 15 e/Å2
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 40
Average electron dose per image: 15 e/Å2
Details: These particles were selected individually.
Final
reconstruction
Resolution: 9.2
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1931
Algorithm: OTHER
Details: A single data set was used for the reconstruction.
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1931
Algorithm: OTHER
Details: A single data set was used for the reconstruction.
⌯ Applied Symmetry
Point group:
I
Software
[1]
Name | Version | Details |
---|---|---|
EMAN2, auto3DEM | - | - |
CTF correction
Details:Auto3DEM
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the EV71 empty capsid.
Details: ::::EMDATABANK.org::::EMD-5466::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the EV71 empty capsid.
Details: ::::EMDATABANK.org::::EMD-5466::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 341 | 341 | 341 |
Origin | -170 | -170 | -170 |
Spacing | 341 | 341 | 341 |
Voxel size | 1.27 Å | 1.27 Å | 1.27 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 1.0 | AUTHOR |