Electron Microscopy Data Bank

EMD-5473

Single-particle
20.0 Å

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Deposition: 19/08/2012
Map released: 02/10/2013
Last modified: 02/10/2013

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5473

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Dynamics in Cryo EM Reconstructions Visualized with Maximum-Likelihood Derived Variance Maps

EMD-5473

Single-particle
20.0 Å
EMD-5473

3D View Gallery

Deposition: 19/08/2012
Map released: 02/10/2013
Last modified: 02/10/2013

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

Method: Single-particle
Aggregation State: Particle

Summary:

Specimen preparation
Microscopy
Image processing
Map

Specimen preparation [1]

Vitrification

Cryogen name: ETHANE
Instrument: FEI VITROBOT MARK I

Microscopy [1]

Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Specimen holder model: GATAN LIQUID NITROGEN

Image Recording [1]

Details: Data were collected using the Leginon automated electron microscopy package and data processing was performed using the Appion pipeline.

Image processing [1]

Details: The reconstruction uses a maximum likelihood algorithm that explicitly takes into account the continuous variability from one instance to another instance of the particle.

Final reconstruction

Resolution: 20.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1200
Algorithm: OTHER
Details:

Applied Symmetry

Point group: I

CTF correction

Details:Each image

Map

Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of fully mature capsid of Nudaurelia capensis omega virus (NwV)
Details: ::::EMDATABANK.org::::EMD-5473::::

Geometry

	X	Y	Z
Dimensions	200	200	200
Origin	-100	-100	-100
Spacing	200	200	200
Voxel size	2.768 Å	2.768 Å	2.768 Å

Contour list

Primary	Level	Source
True	0.0001	AUTHOR