EMD-5495

Single-particle
3.5 Å
EMD-5495
3D View Gallery
Deposition: 12/09/2012
Map released: 24/10/2012
Last modified: 14/11/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5495
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3j26 summary report (pdf.gz) Map and 3j26 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM reconstruction of Sputnik virus

EMD-5495

Single-particle
3.5 Å
EMD-5495
3D View Gallery
Deposition: 12/09/2012
Map released: 24/10/2012
Last modified: 14/11/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 7.0
Details: PBS
Grid
Details: 400 mesh Cu grid (c-flat), 1.2 um hole, glow discharged
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 100 K
Instrument: GATAN CRYOPLUNGE 3
Method: blot 6 seconds
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.1 µm - 2.4 µm
Nominal magnification: 59000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Alignment procedure: LEGACY (Astigmatism: corrected at 250,000 magnification, Electron beam tilt params: )
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Average electron dose per image: 22 e/Å2
Image processing [1]
Details: Using EMAN
Final reconstruction
Resolution: 3.5 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 12000
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EMAN, Frealign - -
⦩ Final angle assignment
Details: EMAN
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of Sputnik virus full particles at 3.5A resolution
Details: ::::EMDATABANK.org::::EMD-5495::::
⬡ Geometry
X Y Z
Dimensions 768 768 384
Origin -384 -384 0
Spacing 768 768 384
Voxel size 1.1 Å 1.1 Å 1.1 Å
Contour list
Primary Level Source
True 2.0 AUTHOR