EMD-5498

Single-particle
18.9 Å
EMD-5498
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Deposition: 12/09/2012
Map released: 17/04/2013
Last modified: 22/05/2013
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EMD-5498
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EMICSS annotations (xml)

Structure of ABCA4, the photoreceptor-specific ATP-binding cassette transporter, in complex with AMPPNP

EMD-5498

Single-particle
18.9 Å
EMD-5498
3D View Gallery
Deposition: 12/09/2012
Map released: 17/04/2013
Last modified: 22/05/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.1 mg/mL
Buffer
pH: 7.5
Details: 20 mM BTP, pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT, 1 mM AMPPNP
Staining
Type: NEGATIVE
Details: Grids with adsorbed protein were stained with 1% w/v uranyl acetate for 10 seconds
Grid
Details: Glow discharged Cu/Pd 400 mesh grids with thin carbon support.
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 0.2 µm - 1.5 µm
Nominal magnification: 50000.0
Calibrated magnification: 70373.0
Specimen holder model: OTHER
Minimum tilt angle: 0
Maximum tilt angle: 45
Image Recording [1]
Number of real images: 200
Image processing [1]
Details: The particles were selected manually
Final reconstruction
Resolution: 18.9 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 38090
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Spider - -
CTF correction
Details:Each defocus group
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the ABCA4-AMPPNP complex
Details: ::::EMDATABANK.org::::EMD-5498::::
⬡ Geometry
X Y Z
Dimensions 144 144 144
Origin 0 0 0
Spacing 144 144 144
Voxel size 2.13 Å 2.13 Å 2.13 Å
Contour list
Primary Level Source
True 0.0363 AUTHOR