EMD-5500
Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM
EMD-5500
Single-particle12.9 Å
Deposition: 28/09/2012
Map released: 16/01/2013
Last modified: 06/03/2013
Buffer
pH: 7.5
Details: 150 mM NH4Cl,10mM Tris-HCL,10mM MgCl2
Details: 150 mM NH4Cl,10mM Tris-HCL,10mM MgCl2
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Blot for 2 seconds before plunging
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Blot for 2 seconds before plunging
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 1.3 µm - 4.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 1.3 µm - 4.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording
[1]
Details: This is a classification result using ML3D methods
Final
reconstruction
Resolution: 12.9
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 32152
Algorithm: OTHER
Details:
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 32152
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
Spider | - | - |
CTF correction
Details:Weiner filter
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D classification of immature ribosomal small subunit from rimm gene deleted E.coli strain
Details: ::::EMDATABANK.org::::EMD-5500::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D classification of immature ribosomal small subunit from rimm gene deleted E.coli strain
Details: ::::EMDATABANK.org::::EMD-5500::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 125 | 125 | 125 |
Origin | -62 | -62 | -62 |
Spacing | 125 | 125 | 125 |
Voxel size | 2.76 Å | 2.76 Å | 2.76 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | -2.8 | AUTHOR |