EMD-5502

Single-particle
13.1 Å
EMD-5502 Deposition: 28/09/2012
Map released: 16/01/2013
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5502

Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM

EMD-5502

Single-particle
13.1 Å
EMD-5502 Deposition: 28/09/2012
Map released: 16/01/2013
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 150mM NH4Cl,10mM Tris-HCL,10mM MgCl2
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Blot for 1 seconds before plunging
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 1.3 µm - 4.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Detector category: CCD
Detector model: GENERIC GATAN (4k x 4k)
Average electron dose per image: 20 e/Å2
Image processing [1]
Details: This is a classification volume (No. 3) using ML3D methods.
Final reconstruction
Resolution: 13.1 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 30262
Algorithm: OTHER
Details:
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Spider - -
CTF correction
Details:Weiner filter
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: The map has been normalized to N(0,1)
Details: ::::EMDATABANK.org::::EMD-5502::::
Geometry
X Y Z
Dimensions 125 125 125
Origin -62 -62 -62
Spacing 125 125 125
Voxel size 2.76 Å 2.76 Å 2.76 Å
Contour list
Primary Level Source
True -2.8 AUTHOR