EMD-5509

Single-particle
16.5 Å
EMD-5509
3D View Gallery
Deposition: 28/09/2012
Map released: 16/01/2013
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5509
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3j2g summary report (pdf.gz) Map and 3j2g FULL report (pdf.gz)
EMICSS annotations (xml)

Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM

EMD-5509

Single-particle
16.5 Å
EMD-5509
3D View Gallery
Deposition: 28/09/2012
Map released: 16/01/2013
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 150 mM NH4Cl,10mM Tris-HCL,10mM MgCl2
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Blot for 1 seconds before plunging
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 1.5 µm - 3.8 µm
Nominal magnification: 59000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Detector category: CCD
Detector model: FEI EAGLE (4k x 4k)
Average electron dose per image: 20 e/Å2
Image processing [1]
Details: This is a classification volume (No. 4) using ML3D methods.
Final reconstruction
Resolution: 16.5 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 25631
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
CTF correction
Details:Weiner filter
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: The map has been normalized to N(0,1)
Details: ::::EMDATABANK.org::::EMD-5509::::
⬡ Geometry
X Y Z
Dimensions 125 125 125
Origin -62 -62 -62
Spacing 125 125 125
Voxel size 3.00 Å 3.00 Å 3.00 Å
Contour list
Primary Level Source
True -2.3 AUTHOR