EMD-5513

Single-particle
11.9 Å
EMD-5513
3D View Gallery
Deposition: 04/10/2012
Map released: 07/11/2012
Last modified: 28/11/2012
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5513
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3j2i summary report (pdf.gz) Map and 3j2i FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of late pre-60S ribosomal subunits with nuclear export factor Arx1 bound at the peptide exit tunnel

EMD-5513

Single-particle
11.9 Å
EMD-5513
3D View Gallery
Deposition: 04/10/2012
Map released: 07/11/2012
Last modified: 28/11/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.0
Details: 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1.5 mM or 5 mM MgCl2, 0.075% (v/v) NP-40
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Instrument: FEI VITROBOT MARK IV
Method: Blot for 2 seconds before plunging
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Calibrated magnification: 95000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: CCD
Detector model: GENERIC CCD
Average electron dose per image: 20 e/Å2
Image processing [1]
Details: The particles were selected using an automatic selection program
Final reconstruction
Resolution: 11.9 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 100000
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Signature, Spider, Chimera - -
CTF correction
Details:Subvolumes
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of pre-60S ribosomal subunits with nuclear export factor Arx1 bound at the peptide exit tunnel
Details: ::::EMDATABANK.org::::EMD-5513::::
⬡ Geometry
X Y Z
Dimensions 368 368 368
Origin -184 -184 -183
Spacing 368 368 368
Voxel size 1.2375 Å 1.2375 Å 1.2375 Å
Contour list
Primary Level Source
True 0.5 EMDB