EMD-5520

Single-particle
3.6 Å
EMD-5520
3D View Gallery
Deposition: 10/10/2012
Map released: 19/12/2012
Last modified: 16/01/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5520
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3j27 summary report (pdf.gz) Map and 3j27 FULL report (pdf.gz)
EMICSS annotations (xml)

CryoEM structure of Dengue virus, full map

EMD-5520

Single-particle
3.6 Å
EMD-5520
3D View Gallery
Deposition: 10/10/2012
Map released: 19/12/2012
Last modified: 16/01/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Details: 50 mM Tris, 140 mM NaCl, 5 mM EDTA
Grid
Details: Quantifoil R2/1
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Method: 2.5 uL sample added per grid
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.5 µm - 2.4 µm
Nominal magnification: 59000.0
Calibrated magnification: 57518.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Alignment procedure: LEGACY (Astigmatism: software compensation, Electron beam tilt params: )
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 1103
Average electron dose per image: 25 e/Å2
Image processing [1]
Details: EMAN with Multi-path Simulated Annealing
Final reconstruction
Resolution: 3.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 9288
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EMAN - -
CTF correction
Details:EMAN per particle, with astigmatism compensation
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Dengue virus capsid, full map
Details: ::::EMDATABANK.org::::EMD-5520::::
⬡ Geometry
X Y Z
Dimensions 480 480 480
Origin -240 -240 -240
Spacing 480 480 480
Voxel size 1.104 Å 1.104 Å 1.104 Å
Contour list
Primary Level Source
True 0.4 AUTHOR