EMD-5525

Single-particle
23.0 Å
EMD-5525
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Deposition: 02/11/2012
Map released: 21/11/2012
Last modified: 14/05/2014
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EMD-5525
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Electron cryo-microscopy of ABC BmrA in 12-fold symmetry rings

EMD-5525

Single-particle
23.0 Å
EMD-5525
3D View Gallery
Deposition: 02/11/2012
Map released: 21/11/2012
Last modified: 14/05/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.1 mg/mL
Buffer
pH: 7.8
Details: 50mM HEPES, 100mM NaCl
Grid
Details: Ted Pella inc. holey formvar grid
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Method: Blot for 3 seconds before plunging.
Details: Specimen linked to a Ni++-NTA-DOGS/DOPC lipid monolayer
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.26 mm
Nominal defocus: 2.6 µm - 5.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON COOLSCAN
Number of real images: 102
Average electron dose per image: 15 e/Å2
Bits per pixel: 8.0
Image processing [1]
Details: Xmipp CL2D classification and Projection matching refinement with Invert Fourier 3D reconstruction
Final reconstruction
Resolution: 23.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1188
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: D12
Software [1]
Name Version Details
Xmipp - -
CTF correction
Details:Micrograph based
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of BmrA in a lipidic environment. Reconstruction is D12 symmetrized. 24 homodimers of BmrA are inserted into a lipid bilayer.
Details: ::::EMDATABANK.org::::EMD-5525::::
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin 0 0 0
Spacing 200 200 200
Voxel size 2.50 Å 2.50 Å 2.50 Å
Contour list
Primary Level Source
True 0.029 AUTHOR