EMD-5527

Single-particle
17.5 Å
EMD-5527
3D View Gallery
Deposition: 02/11/2012
Map released: 17/04/2013
Last modified: 17/04/2013
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EMD-5527
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EMICSS annotations (xml)

Reconstruction of the deltaGCC mutant HCV IRES bound to rabbit 40S ribosomal subunit

EMD-5527

Single-particle
17.5 Å
EMD-5527
3D View Gallery
Deposition: 02/11/2012
Map released: 17/04/2013
Last modified: 17/04/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Details: 20 mM Tris-HCl, pH 7.4, 200 mM KOAc, 2.5 mM MgCl2, 2 mM DTT, 40 mM KCl
Grid
Details: Quantifoil R1.2/1.3 400 mesh copper grids with holey carbon
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Blot and plunge into liquid ethane
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.25 µm - 5.0 µm
Nominal magnification: 62000.0
Calibrated magnification: 117293.0
Specimen holder model: GATAN LIQUID NITROGEN
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: CCD
Detector model: GENERIC TVIPS (4k x 4k)
Number of real images: 1790
Average electron dose per image: 15 e/Å2
Details: Used 2x binned images yielding a pixel size of 2.66 A/pixel.
Image processing [1]
Details: Particles selected manually using Electron Micrographe Utility (cryoem.ucsf.edu)
Final reconstruction
Resolution: 17.5 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 29920
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
FREALIGN - -
CTF correction
Details:CTFFIND3
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of mutant HCV IRES bound to 40S
Details: ::::EMDATABANK.org::::EMD-5527::::
⬡ Geometry
X Y Z
Dimensions 150 150 150
Origin 0 0 0
Spacing 150 150 150
Voxel size 2.66 Å 2.66 Å 2.66 Å
Contour list
Primary Level Source
True 2.25 AUTHOR