EMD-5528

Single-particle
25.0 Å
EMD-5528 Deposition: 02/11/2012
Map released: 06/03/2013
Last modified: 15/05/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5528

Cryo-EM structure of the contracted bacteriophage T4 tail containing the collar and whiskers made of fibritin molecules.

EMD-5528

Single-particle
25.0 Å
EMD-5528 Deposition: 02/11/2012
Map released: 06/03/2013
Last modified: 15/05/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Details: 50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 8 mM MgCl2
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.5 µm - 3.0 µm
Nominal magnification: 38000.0
Calibrated magnification: 39190.0
Specimen holder model: GATAN LIQUID NITROGEN
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 97
Average electron dose per image: 16 e/Å2
Bits per pixel: 8.0
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 2727
Algorithm: OTHER
Applied Symmetry
Point group: C6
Software [1]
Name Version Details
EMAN - -
CTF correction
Details:Phase flipping
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of the contracted T4 tail containing the collar and whiskers
Details: ::::EMDATABANK.org::::EMD-5528::::
Geometry
X Y Z
Dimensions 250 250 250
Origin -125 -125 -125
Spacing 250 250 250
Voxel size 3.572 Å 3.572 Å 3.572 Å
Contour list
Primary Level Source
True 2.0 AUTHOR