EMD-5528
Cryo-EM structure of the contracted bacteriophage T4 tail containing the collar and whiskers made of fibritin molecules.
EMD-5528
Single-particle25.0 Å
Deposition: 02/11/2012
Map released: 06/03/2013
Last modified: 15/05/2013
Buffer
pH: 8.0
Details: 50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 8 mM MgCl2
Details: 50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 8 mM MgCl2
Vitrification
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.5 µm - 3.0 µm
Nominal magnification: 38000.0
Calibrated magnification: 39190.0
Specimen holder model: GATAN LIQUID NITROGEN
Minimum tilt angle: 0
Maximum tilt angle: 0
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.5 µm - 3.0 µm
Nominal magnification: 38000.0
Calibrated magnification: 39190.0
Specimen holder model: GATAN LIQUID NITROGEN
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 97
Average electron dose per image: 16 e/Å2
Bits per pixel: 8.0
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 97
Average electron dose per image: 16 e/Å2
Bits per pixel: 8.0
Final
reconstruction
Resolution: 25.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 2727
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 2727
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
C6
Software
[1]
Name | Version | Details |
---|---|---|
EMAN | - | - |
CTF correction
Details:Phase flipping
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of the contracted T4 tail containing the collar and whiskers
Details: ::::EMDATABANK.org::::EMD-5528::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of the contracted T4 tail containing the collar and whiskers
Details: ::::EMDATABANK.org::::EMD-5528::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 250 | 250 | 250 |
Origin | -125 | -125 | -125 |
Spacing | 250 | 250 | 250 |
Voxel size | 3.572 Å | 3.572 Å | 3.572 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 2.0 | AUTHOR |