EMD-5561
Cryo EM map of the Gaalphaq-PLCbeta3 complex.
EMD-5561
Single-particle19.0 Å
Deposition: 03/01/2013
Map released: 20/03/2013
Last modified: 17/04/2013
Concentration: 1
mg/mL
Buffer
pH: 8.0
Details: 20 mM HEPES, pH 8, 200 mM NaCl, 2 mM DTT, 0.9 mM CaCl2, 50 uM GDP, 30 uM AlCl3, 10 mM NaF, 5 mM MgCl2
Details: 20 mM HEPES, pH 8, 200 mM NaCl, 2 mM DTT, 0.9 mM CaCl2, 50 uM GDP, 30 uM AlCl3, 10 mM NaF, 5 mM MgCl2
Grid
Details: Glow-discharged Quantifoil R2/200 mesh grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Blot 3 microliter sample for 2 seconds.
Details: Vitrification carried out in liquid nitrogen atmosphere.
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Blot 3 microliter sample for 2 seconds.
Details: Vitrification carried out in liquid nitrogen atmosphere.
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2 mm
Nominal defocus: 1.5 µm - 3.5 µm
Nominal magnification: 50000.0
Calibrated magnification: 71138.0
Specimen holder model: OTHER
Specimen holder details: Used two holders.
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 135,000 times magnification., Electron beam tilt params: )
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2 mm
Nominal defocus: 1.5 µm - 3.5 µm
Nominal magnification: 50000.0
Calibrated magnification: 71138.0
Specimen holder model: OTHER
Specimen holder details: Used two holders.
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 135,000 times magnification., Electron beam tilt params: )
Temperature
Minimum: 89
K
Average: 89 K
Maximum: 95 K
Average: 89 K
Maximum: 95 K
Image Recording
[1]
Detector category:
CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of real images: 350
Average electron dose per image: 20 e/Å2
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of real images: 350
Average electron dose per image: 20 e/Å2
Final
reconstruction
Resolution: 19.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 12692
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 12692
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
EMAN1 | - | - |
CTF correction
Details:Each particle
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the PLCbeta3 distal CTD in complex with the N-terminal helix of Galphaq
Details: ::::EMDATABANK.org::::EMD-5561::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the PLCbeta3 distal CTD in complex with the N-terminal helix of Galphaq
Details: ::::EMDATABANK.org::::EMD-5561::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 64 | 64 | 64 |
Origin | -32 | -32 | -32 |
Spacing | 64 | 64 | 64 |
Voxel size | 4.48 Å | 4.48 Å | 4.48 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 2.5 | AUTHOR |