EMD-5565
CS-tubulin Kinesin-13 Microtubule complex
EMD-5565
Helical reconstruction10.8 Å
Deposition: 09/01/2013
Map released: 06/03/2013
Last modified: 10/04/2013
Vitrification
Microscope: JEOL 3200FSC
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: JEOL
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: JEOL
Image Recording
[1]
Scanner:
OTHER
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Gatan 626
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Gatan 626
Image Recording
[1]
Scanner:
OTHER
Details: Custom single particle alignment method based on IHRSR
Final
reconstruction
Resolution: 10.8
Å
(
BY AUTHOR)
Resolution method: OTHER
Details:
Resolution method: OTHER
Details:
⌯ Applied Symmetry
ΔΖ:
5.55267
Å
ΔΦ: 168.08743°
ΔΦ: 168.08743°
Software
[1]
Name | Version | Details |
---|---|---|
Spider, IHRSR, CTFFIND3, Custom | - | - |
CTF correction
Details:Each particle
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the kinesin-13 KLP10A head domain in complex with CS-tubulin and a microtubule
Details: ::::EMDATABANK.org::::EMD-5565::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the kinesin-13 KLP10A head domain in complex with CS-tubulin and a microtubule
Details: ::::EMDATABANK.org::::EMD-5565::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 290 | 290 | 80 |
Origin | 0 | 0 | 0 |
Spacing | 290 | 290 | 80 |
Voxel size | 2 Å | 2 Å | 2 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.015 | EMDB |