EMD-5581

Single-particle
23.0 Å
EMD-5581
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Deposition: 31/01/2013
Map released: 13/02/2013
Last modified: 13/02/2013
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EMD-5581
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EMICSS annotations (xml)

Negatively Stained TEM structure of trypanosomatid core editing complex (L-complex)

EMD-5581

Single-particle
23.0 Å
EMD-5581
3D View Gallery
Deposition: 31/01/2013
Map released: 13/02/2013
Last modified: 13/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.6
Details: 20 mM Tris, pH 7.6, 60 mM KCl, 10 mM MgCl2
Staining
Type: NEGATIVE
Details: 2% uranyl acetate for 2 min
Grid
Details: Continuous carbon grid
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 1.6 µm - 2.1 µm
Nominal magnification: 70000.0
Calibrated magnification: 68200.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Specimen holder details: Single tilt room temperature holder
Temperature
Average: 300 K
Image Recording [1]
Detector category: CCD
Detector model: GENERIC CCD
Sampling interval: 15 µm
Number of real images: 26
Average electron dose per image: 400 e/Å2
Image processing [1]
Final reconstruction
Resolution: 23.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 12500
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
EMAN - -
Final 2D classification
Number of classes: 879
CTF correction
Details:Phase flip
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of negatively stained L-complex
Details: ::::EMDATABANK.org::::EMD-5581::::
⬡ Geometry
X Y Z
Dimensions 192 192 192
Origin -44 -44 -44
Spacing 192 192 192
Voxel size 2.2 Å 2.2 Å 2.2 Å
Contour list
Primary Level Source
True 5.82 AUTHOR