EMD-5584

Single-particle
4.5 Å
EMD-5584
3D View Gallery
Deposition: 18/02/2013
Map released: 01/05/2013
Last modified: 20/11/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5584
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3j31 summary report (pdf.gz) Map and 3j31 FULL report (pdf.gz)
EMICSS annotations (xml)

Life in the extremes: atomic structure of Sulfolobus Turreted Icosahedral Virus

EMD-5584

Single-particle
4.5 Å
EMD-5584
3D View Gallery
Deposition: 18/02/2013
Map released: 01/05/2013
Last modified: 20/11/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 3.5
Details: 23 mM KH2PO4, 19 mM (NH4)2SO4, 1 mM MgSO4, 2 mM CaCl2
Grid
Details: Glow-discharged C-flat holey carbon grids (CF-2/2-4C, Protochips)
Vitrification
Cryogen name: ETHANE
Chamber temperature: 94 K
Instrument: HOMEMADE PLUNGER
Details: Vitrification carried out at room temperature
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.8 µm - 2.5 µm
Calibrated magnification: 102189.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Specimen holder details: Nitrogen cooled
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 103,000 times magnification, Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 0
Details: Nanoprobe mode was used
Temperature
Average: 90 K
Image Recording [1]
Detector category: CCD
Detector model: FEI FALCON I (4k x 4k)
Number of real images: 878
Average electron dose per image: 18 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.5 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 8903
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
Frealign - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of mature STIV
Details: ::::EMDATABANK.org::::EMD-5584::::
⬡ Geometry
X Y Z
Dimensions 1024 1024 1024
Origin -512 -512 -512
Spacing 1024 1024 1024
Voxel size 1.37 Å 1.3700001 Å 1.37 Å
Contour list
Primary Level Source
True 0.05 AUTHOR