EMD-5591

Single-particle
6.0 Å
EMD-5591
3D View Gallery
Deposition: 28/02/2013
Map released: 01/05/2013
Last modified: 23/07/2014
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5591
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Electron cryo-microscopy of Drosophila melanogaster EF2- and Vig2-bound 80S ribosome

EMD-5591

Single-particle
6.0 Å
EMD-5591
3D View Gallery
Deposition: 28/02/2013
Map released: 01/05/2013
Last modified: 23/07/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: Before plunging, blot for 3 seconds using two layers of filter paper.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.0 µm - 4.0 µm
Nominal magnification: 90000.0
Calibrated magnification: 90000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Detector category: CCD
Detector model: FEI EAGLE (4k x 4k)
Average electron dose per image: 20 e/Å2
Image processing [1]
Details: The particles were selected using an automatic selection program
Final reconstruction
Resolution: 6.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 134500
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Spider - -
CTF correction
Details:Each subvolume
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of D. melanogaster 80S ribosome
Details: ::::EMDATABANK.org::::EMD-5591::::
⬡ Geometry
X Y Z
Dimensions 368 368 368
Origin -184 -184 -183
Spacing 368 368 368
Voxel size 1.2375 Å 1.2375 Å 1.2375 Å
Contour list
Primary Level Source
True 0.49 AUTHOR