EMD-5607

Single-particle
9.0 Å
EMD-5607
3D View Gallery
Deposition: 20/03/2013
Map released: 15/05/2013
Last modified: 28/08/2013
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5607
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Structural dynamics and inter-ring communication of the MecA-ClpC complex during active substrate unfolding and translocation revealed by cryo-EM

EMD-5607

Single-particle
9.0 Å
EMD-5607
3D View Gallery
Deposition: 20/03/2013
Map released: 15/05/2013
Last modified: 28/08/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.03 mg/mL
Buffer
pH: 7.5
Details: 50mM kCl, 10mM Tris-HCL,2mM MgCl2, 2mM ATP
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 90 K
Instrument: FEI VITROBOT MARK IV
Method: Blot for 2 seconds before plunging
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 1.5 µm - 3.0 µm
Nominal magnification: 59000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: CCD
Detector model: FEI EAGLE (4k x 4k)
Number of real images: 994
Average electron dose per image: 20 e/Å2
Image processing [1]
Final reconstruction
Resolution: 9.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 45514
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C6
Software [1]
Name Version Details
SPIDER - -
CTF correction
Details:Each defocus group on 3D level
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of MecA-ClpC(E280A) with ATP, MW-ATP
Details: ::::EMDATABANK.org::::EMD-5607::::
⬡ Geometry
X Y Z
Dimensions 150 150 150
Origin -74 -74 -74
Spacing 150 150 150
Voxel size 1.5 Å 1.5 Å 1.5 Å
Contour list
Primary Level Source
True 1.5 AUTHOR