EMD-5612

Single-particle
7.7 Å
EMD-5612 Deposition: 21/03/2013
Map released: 15/05/2013
Last modified: 05/06/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5612

Yeast 20S proteasome with C-terminal peptide of yeast Rpt2

EMD-5612

Single-particle
7.7 Å
EMD-5612 Deposition: 21/03/2013
Map released: 15/05/2013
Last modified: 05/06/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Grid
Details: Quantifoil grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 120 K
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.2 µm - 2.1 µm
Nominal magnification: 100000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: CT3500
Minimum tilt angle: 0
Maximum tilt angle: 0
Details: Low-Dose procedure
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F816 (8k x 8k)
Average electron dose per image: 25 e/Å2
Image processing [1]
Details: FREALIGN
Final reconstruction
Resolution: 7.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 88013
Details:
Applied Symmetry
Point group: C2
Software [1]
Name Version Details
FREALIGN - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D density map of yeast 20S proteasome core particle with GST tag added to the C-terminus of beta-2 subunit in complex with the C-terminal peptide of yeast Rpt2
Details: ::::EMDATABANK.org::::EMD-5612::::
Geometry
X Y Z
Dimensions 200 200 200
Origin 0 0 0
Spacing 200 200 200
Voxel size 1.47 Å 1.47 Å 1.47 Å
Contour list
Primary Level Source
True 0.8 AUTHOR