EMD-5613
Yeast 20S proteasome with C-terminal peptide of yeast Rpt3
EMD-5613
Single-particle7.7 Å
Deposition: 21/03/2013
Map released: 15/05/2013
Last modified: 05/06/2013
Grid
Details: Quantifoil grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 120 K
Instrument: FEI VITROBOT MARK III
Chamber humidity: 100%
Chamber temperature: 120 K
Instrument: FEI VITROBOT MARK III
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.2 µm - 2.1 µm
Nominal magnification: 100000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: CT3500
Minimum tilt angle: 0
Maximum tilt angle: 0
Details: Low-Dose procedure
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.2 µm - 2.1 µm
Nominal magnification: 100000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: CT3500
Minimum tilt angle: 0
Maximum tilt angle: 0
Details: Low-Dose procedure
Image Recording
[1]
Detector category:
CCD
Detector model: TVIPS TEMCAM-F816 (8k x 8k)
Average electron dose per image: 25 e/Å2
Detector model: TVIPS TEMCAM-F816 (8k x 8k)
Average electron dose per image: 25 e/Å2
Details: FREALIGN
Final
reconstruction
Resolution: 7.7
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 74177
Details:
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 74177
Details:
⌯ Applied Symmetry
Point group:
C2
Software
[1]
Name | Version | Details |
---|---|---|
FREALIGN | - | - |
CTF correction
Details:Each particle
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D density map of yeast 20S proteasome core particle with GST tag added to the C-terminus of beta-2 subunit in complex with the C-terminal peptide of yeast Rpt3
Details: ::::EMDATABANK.org::::EMD-5613::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D density map of yeast 20S proteasome core particle with GST tag added to the C-terminus of beta-2 subunit in complex with the C-terminal peptide of yeast Rpt3
Details: ::::EMDATABANK.org::::EMD-5613::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 200 | 200 | 200 |
Origin | 0 | 0 | 0 |
Spacing | 200 | 200 | 200 |
Voxel size | 1.47 Å | 1.47 Å | 1.47 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.8 | AUTHOR |