EMD-5617

Single-particle
9.6 Å
EMD-5617
3D View Gallery
Deposition: 21/03/2013
Map released: 15/05/2013
Last modified: 05/06/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5617
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Yeast 20S proteasome in complex with purified yeast 19S base sub complex

EMD-5617

Single-particle
9.6 Å
EMD-5617
3D View Gallery
Deposition: 21/03/2013
Map released: 15/05/2013
Last modified: 05/06/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 120 K
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.2 µm - 2.1 µm
Nominal magnification: 100000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: CT3500
Minimum tilt angle: 0
Maximum tilt angle: 0
Details: Low-dose procedure
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F816 (8k x 8k)
Average electron dose per image: 25 e/Å2
Image processing [1]
Details: FREALIGN
Final reconstruction
Resolution: 9.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 51000
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
FREALIGN - -
CTF correction
Details:Each Particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D density map of yeast 20S proteasome core particle with GST tag in complex with purified yeast 19S base subcomplex
Details: ::::EMDATABANK.org::::EMD-5617::::
⬡ Geometry
X Y Z
Dimensions 280 280 280
Origin 0 0 0
Spacing 280 280 280
Voxel size 1.47 Å 1.47 Å 1.47 Å
Contour list
Primary Level Source
True 0.45 AUTHOR