EMD-5630
Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgC mutant
EMD-5630
Subtomogram averaging38.0 Å
Deposition: 05/04/2013
Map released: 14/08/2013
Last modified: 26/03/2014
Buffer
Details: BSK-II liquid medium supplemented with 6% rabbit serum
Grid
Details: Freshly glow-discharged holey carbon grids
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Method: Bacterial culture was deposited onto freshly glow-discharged, holey carbon grids for 1 min. Grids were blotted with filter paper and then rapidly frozen in liquid ethane, using a homemade gravity-driven plunger apparatus.
Instrument: HOMEMADE PLUNGER
Method: Bacterial culture was deposited onto freshly glow-discharged, holey carbon grids for 1 min. Grids were blotted with filter paper and then rapidly frozen in liquid ethane, using a homemade gravity-driven plunger apparatus.
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 6.0 µm - 8.0 µm
Nominal magnification: 31000.0
Specimen holder model: OTHER
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 6.0 µm - 8.0 µm
Nominal magnification: 31000.0
Specimen holder model: OTHER
Temperature
Minimum: 170
K
Image Recording
[1]
Number of real images: 21315
Average electron dose per image: 100 e/Å2
Average electron dose per image: 100 e/Å2
Tilt Series
[1]
Axis 1 | Axis 2 | |||||
---|---|---|---|---|---|---|
Min. | Max. | Inc. | Min. | Max. | Inc. | Rotation |
-65 ° | 65 ° | - | - | - | - | - |
Details: The subvolumes of the flagellar motors were extracted computationally from the tomograms and were further aligned in 3D.
Final
reconstruction
Resolution: 38.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
IMOD, Raptor, Protomo | - | - |
Final 3D classification
Number of classes:
4
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Flagellar motor of a FlgC mutant in B. burdorferi
Details: ::::EMDATABANK.org::::EMD-5630::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Flagellar motor of a FlgC mutant in B. burdorferi
Details: ::::EMDATABANK.org::::EMD-5630::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 256 | 256 | 256 |
Origin | -7 | -7 | -7 |
Spacing | 256 | 256 | 256 |
Voxel size | 5.7 Å | 5.7 Å | 5.7 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 2.5 | AUTHOR |