EMD-5656

Single-particle
14.7 Å
EMD-5656
3D View Gallery
Deposition: 01/05/2013
Map released: 10/07/2013
Last modified: 04/09/2013
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5656
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EMICSS annotations (xml)

Pseudorabies GS-2168 B-capsid from cryo-electron microscopy

EMD-5656

Single-particle
14.7 Å
EMD-5656
3D View Gallery
Deposition: 01/05/2013
Map released: 10/07/2013
Last modified: 04/09/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: TNE: 500mM NaCl, 10mM Tris, 1mM EDTA
Grid
Details: Quantifoil R2/1, 200 mesh copper, glow discharged for 10-15 secs
Vitrification
Cryogen name: ETHANE-PROPANE MIXTURE
Chamber humidity: 95%
Chamber temperature: 82 K
Instrument: FEI VITROBOT MARK III
Method: Blot for 6 seconds.
Microscopy [1]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal magnification: 29000.0
Calibrated magnification: 30000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Gatan 626
Temperature
Maximum: 95 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 41
Bits per pixel: 8.0
Image processing [1]
Details: Particles were picked with x3dpreprocess.
Final reconstruction
Resolution: 14.7 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 5870
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
AUTO3DEM(v3.07) - -
CTF correction
Details:Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Reconstruction of PRV GS-2168 UL25-null B-capsid
Details: ::::EMDATABANK.org::::EMD-5656::::
⬡ Geometry
X Y Z
Dimensions 723 723 723
Origin 0 0 0
Spacing 723 723 723
Voxel size 2.12 Å 2.12 Å 2.12 Å
Contour list
Primary Level Source
True 2500.0 AUTHOR