EMD-5764

Single-particle
3.5 Å
EMD-5764
3D View Gallery
Deposition: 09/10/2013
Map released: 25/12/2013
Last modified: 08/01/2014
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EMD-5764
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5A resolution

EMD-5764

Single-particle
3.5 Å
EMD-5764
3D View Gallery
Deposition: 09/10/2013
Map released: 25/12/2013
Last modified: 08/01/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 50 mM Tris-HCl, 250 mM NaCl
Grid
Details: 400 mesh holey carbon film
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.8 µm - 2.3 µm
Nominal magnification: 59000.0
Calibrated magnification: 57660.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Alignment procedure: LEGACY (Astigmatism: manual correction, Electron beam tilt params: 0)
Temperature
Average: 80 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 895
Average electron dose per image: 25 e/Å2
Bits per pixel: 2.0
Image processing [1]
Final reconstruction
Resolution: 3.5 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 39549
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
ELite3D, Frealign - -
Final 2D classification
Number of classes: 1
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3.5A cryoEM structure of Bordetella bacteriophage
Details: ::::EMDATABANK.org::::EMD-5764::::
⬡ Geometry
X Y Z
Dimensions 680 680 680
Origin -340 -340 -340
Spacing 680 680 680
Voxel size 1.1 Å 1.1 Å 1.1 Å
Contour list
Primary Level Source
True 0.025 AUTHOR