› 11ba

X-ray diffraction
2.06Å resolution

BINDING OF A SUBSTRATE ANALOGUE TO A DOMAIN SWAPPING PROTEIN IN THE COMPLEX OF BOVINE SEMINAL RIBONUCLEASE WITH URIDYLYL-2',5'-ADENOSINE

Released:
DOI: 10.2210/pdb11ba/pdb
PDB entry 11ba coloured by chain, front view.
PDB entry 11ba coloured by chain, side view.
PDB entry 11ba coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Binding of a substrate analog to a domain swapping protein: X-ray structure of the complex of bovine seminal ribonuclease with uridylyl(2',5')adenosine.
Vitagliano L, Adinolfi S, Riccio A, Sica F, Zagari A, Mazzarella L
Protein Sci 7 1691-9 (1998)
PMID: 10082366

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seminal ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.63 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00669 (Residues: 27-150; Coverage: 100%)
Gene name: SRN
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand UPA 2 x UPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 49.45Å b: 60.64Å c: 50.75Å
α: 90° β: 117.35° γ: 90°
R-values:
R R work R free
0.184 0.184 not available

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Citations

17 citation in other articles

Subtle functional collective motions in pancreatic-like ribonucleases: from ribonuclease A to angiogenin.
Merlino et al. (2003)
16 more

13 mentions without citation

Effects of manure compost application on soil microbial community diversity and soil microenvironments in a temperate cropland in China.
Zhen et al. (2014)
12 more