› 11bg

X-ray diffraction
1.9Å resolution

A POTENTIAL ALLOSTERIC SUBSITE GENERATED BY DOMAIN SWAPPING IN BOVINE SEMINAL RIBONUCLEASE

Released:
DOI: 10.2210/pdb11bg/pdb
PDB entry 11bg coloured by chain, front view.
PDB entry 11bg coloured by chain, side view.
PDB entry 11bg coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease.
Vitagliano L, Adinolfi S, Sica F, Merlino A, Zagari A, Mazzarella L
J Mol Biol 293 569-77 (1999)
PMID: 10543951

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-132818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seminal ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.63 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00669 (Residues: 27-150; Coverage: 100%)
Gene name: SRN
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand U2G 4 x U2G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Unit cell:
a: 36.4Å b: 66.7Å c: 107.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 not available

Quick links

Citations

4 review citations

The unfolding story of three-dimensional domain swapping.
Rousseau et al. (2003)
3 more

7 mentions without citation

Protein deamidation.
Robinson NE. (2002)
6 more