› 124d

Solution NMR

STRUCTURE OF A DNA:RNA HYBRID DUPLEX: WHY RNASE H DOES NOT CLEAVE PURE RNA

Released:
DOI: 10.2210/pdb124d/pdb
PDB entry 124d coloured by chain, front view.
PDB entry 124d coloured by chain, side view.
PDB entry 124d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structure of a DNA:RNA hybrid duplex. Why RNase H does not cleave pure RNA.
Salazar M, Reid BR
J Mol Biol 233 509-23 (1993)
PMID: 8411159

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113315 (preferred)
Entry contents:
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
DNA (5'-D(*GP*TP*CP*AP*CP*AP*TP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*CP*AP*UP*GP*UP*GP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.53 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: RESTRAINED MOLECULAR DYNAMICS/NOESY SIMULATION REFINEMENT
Expression system: Not provided

Quick links

Citations

19 review citations

LNA: a versatile tool for therapeutics and genomics.
Petersen et al. (2003)
18 more

5 mentions without citation

The mechanism of ATP-dependent primer-template recognition by a clamp loader complex.
Simonetta et al. (2009)
4 more