› 145d

X-ray diffraction
1.25Å resolution

Structure and thermodynamics of nonalternating C/G base pairs in Z-DNA: the 1.3 angstroms crystal structure of the asymmetric hexanucleotide D(M(5)CGGGM(5) CG)/D(M(5)CGCCM(5)CG)

Released:
DOI: 10.2210/pdb145d/pdb
PDB entry 145d coloured by chain, front view.
PDB entry 145d coloured by chain, side view.
PDB entry 145d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structure and thermodynamics of nonalternating C.G base pairs in Z-DNA: the 1.3-A crystal structure of the asymmetric hexanucleotide d(m5CGGGm5CG).d(m5CGCCm5CG).
Schroth GP, Kagawa TF, Ho PS
Biochemistry 32 13381-92 (1993)
PMID: 8257675

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218896 (preferred)
Entry contents:
3 distinct DNA molecules
Macromolecules (3 distinct):
DNA (5'-D(*(MCY)P*GP*GP*GP*(5CM)P*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 6 nucleotides
Theoretical weight: 1.8 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*(MCY)P*GP*CP*CP*(5CM)P*G)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 6 nucleotides
Theoretical weight: 1.72 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*(5CM)P*DGP*DGP*DGP*(5CM)P*DG)-3') Chain: D
Molecule details ›
Chain: D
Length: 6 nucleotides
Theoretical weight: 1.88 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MCY 3 x MCY
Ligand 5CM 5 x 5CM

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 17.865Å b: 30.822Å c: 44.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 not available not available
Expression system: Not provided

Quick links

Citations

4 review citations

DNA structural forms.
Hartmann et al. (1996)
3 more

4 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
3 more