› 16pk

X-ray diffraction
1.6Å resolution

PHOSPHOGLYCERATE KINASE FROM TRYPANOSOMA BRUCEI BISUBSTRATE ANALOG

Released:
DOI: 10.2210/pdb16pk/pdb
PDB entry 16pk coloured by chain, front view.
PDB entry 16pk coloured by chain, side view.
PDB entry 16pk coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei.
Bernstein BE, Williams DM, Bressi JC, Kuhn P, Gelb MH, Blackburn GM, Hol WG
J Mol Biol 279 1137-48 (1998)
PMID: 9642090

Function and Biology Details

Reaction catalysed: 
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139573 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycerate kinase, glycosomal Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 44.7 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07378 (Residues: 5-419; Coverage: 94%)
Sequence domains: Phosphoglycerate kinase
Structure domains: Phosphoglycerate kinase, N-terminal domain

Ligands and Environments

2 bound ligands:
Ligand BIS 1 x BIS
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 72.71Å b: 79.76Å c: 81.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.235
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

ATP synthesis driven by proton transport in F1F0-ATP synthase.
Weber et al. (2003)
5 more

15 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
14 more