› 1ous

X-ray diffraction
1.2Å resolution

Lecb (PA-LII) calcium-free

Released:
DOI: 10.2210/pdb1ous/pdb
PDB entry 1ous coloured by chain, front view.
PDB entry 1ous coloured by chain, side view.
PDB entry 1ous coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa.
Loris R, Tielker D, Jaeger KE, Wyns L
J Mol Biol 331 861-70 (2003)
PMID: 12909014

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 114 amino acids
Theoretical weight: 11.73 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HYN5 (Residues: 2-115; Coverage: 99%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 46.81Å b: 73.8Å c: 100.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.209
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structures of the lectins from Pseudomonas aeruginosa: insight into the molecular basis for host glycan recognition.
Imberty et al. (2004)
8 more

6 mentions without citation

Building macromolecular assemblies by information-driven docking: introducing the HADDOCK multibody docking server.
Karaca et al. (2010)
5 more