› 1oxc

X-ray diffraction
1.2Å resolution

LecB (PA-LII) in complex with FUCOSE

Released:
DOI: 10.2210/pdb1oxc/pdb
PDB entry 1oxc coloured by chain, front view.
PDB entry 1oxc coloured by chain, side view.
PDB entry 1oxc coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa.
Loris R, Tielker D, Jaeger KE, Wyns L
J Mol Biol 331 861-70 (2003)
PMID: 12909014

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 114 amino acids
Theoretical weight: 11.73 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HYN5 (Residues: 2-115; Coverage: 99%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

4 bound ligands:
Ligand FUL 2 x FUL
Ligand FUC 4 x FUC
Ligand CA 8 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 49.088Å b: 73.058Å c: 99.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.185
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structures of the lectins from Pseudomonas aeruginosa: insight into the molecular basis for host glycan recognition.
Imberty et al. (2004)
8 more

8 mentions without citation

The virulence factor LecB varies in clinical isolates: consequences for ligand binding and drug discovery.
Sommer et al. (2016)
7 more