› 1w8f

X-ray diffraction
1.05Å resolution

PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL)COMPLEXED WITH LACTO-N-NEO- FUCOPENTAOSE V(LNPFV)

Released:
DOI: 10.2210/pdb1w8f/pdb
PDB entry 1w8f coloured by chain, front view.
PDB entry 1w8f coloured by chain, side view.
PDB entry 1w8f coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa.
Perret S, Sabin C, Dumon C, Pokorná M, Gautier C, Galanina O, Ilia S, Bovin N, Nicaise M, Desmadril M, Gilboa-Garber N, Wimmerová M, Mitchell EP, Imberty A
Biochem J 389 325-32 (2005)
PMID: 15790314

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 115 amino acids
Theoretical weight: 11.87 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HYN5 (Residues: 1-115; Coverage: 100%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, GAL, NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, GAL, FUC
Carbohydrate polymer
3 bound ligands:
Ligand SO4 4 x SO4
Ligand CA 8 x CA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 47.015Å b: 48.352Å c: 52.472Å
α: 85.35° β: 80.05° γ: 65.6°
R-values:
R R work R free
0.106 0.105 0.123
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

From carbohydrate leads to glycomimetic drugs.
Ernst et al. (2009)
13 more

2 mentions without citation

Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa.
Perret et al. (2005)
1 more