› 1w8h

X-ray diffraction
1.75Å resolution

structure of pseudomonas aeruginosa lectin II (PA-IIL)complexed with lewisA trisaccharide

Released:
DOI: 10.2210/pdb1w8h/pdb
PDB entry 1w8h coloured by chain, front view.
PDB entry 1w8h coloured by chain, side view.
PDB entry 1w8h coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa.
Perret S, Sabin C, Dumon C, Pokorná M, Gautier C, Galanina O, Ilia S, Bovin N, Nicaise M, Desmadril M, Gilboa-Garber N, Wimmerová M, Mitchell EP, Imberty A
Biochem J 389 325-32 (2005)
PMID: 15790314

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 115 amino acids
Theoretical weight: 11.87 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HYN5 (Residues: 1-115; Coverage: 100%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, NDG, FUC
Ligand NAG 1 x NAG
Ligand GAL 1 x GAL
Ligand NDG 1 x NDG
Ligand FUC 1 x FUC
Carbohydrate polymer : NEW Components: NAG, GAL, NDG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, NDG, FUC
Carbohydrate polymer
3 bound ligands:
Ligand CA 8 x CA
Ligand SO4 2 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 51.575Å b: 75.824Å c: 52.746Å
α: 90° β: 93.48° γ: 90°
R-values:
R R work R free
0.126 0.125 0.169
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

From carbohydrate leads to glycomimetic drugs.
Ernst et al. (2009)
13 more

6 mentions without citation

Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa.
Perret et al. (2005)
5 more