› 1a0a

X-ray diffraction
2.8Å resolution

PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX

Released:
DOI: 10.2210/pdb1a0a/pdb
PDB entry 1a0a coloured by chain, front view.
PDB entry 1a0a coloured by chain, side view.
PDB entry 1a0a coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition.
Shimizu T, Toumoto A, Ihara K, Shimizu M, Kyogoku Y, Ogawa N, Oshima Y, Hakoshima T
EMBO J 16 4689-97 (1997)
PMID: 9303313

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113379 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Phosphate system positive regulatory protein PHO4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07270 (Residues: 250-312; Coverage: 20%)
Gene names: PHO4, YFR034C
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
DNA (5'-D(*CP*TP*CP*AP*CP*AP*CP*GP*TP*GP*GP*GP*AP*CP*TP*AP*G )-3') Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.21 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
DNA (5'-D(*CP*TP*AP*GP*TP*CP*CP*CP*AP*CP*GP*TP*GP*TP*GP*AP*G )-3') Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P212121
Unit cell:
a: 53.51Å b: 68.3Å c: 108.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.284
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

The phosphatase system in Saccharomyces cerevisiae.
Oshima Y. (1997)
9 more

15 mentions without citation

Absence of a simple code: how transcription factors read the genome.
Slattery et al. (2014)
14 more